Random Mutagenesis of the M3 Muscarinic Acetylcholine Receptor Expressed in Yeast
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چکیده
منابع مشابه
Molecular cloning of m3 muscarinic acetylcholine receptor in rat iris.
Muscarinic receptor subtypes that involved in cholinergic responses in rat iris were identified by reverse transcription-polymerase chain reaction (RT-PCR) analysis. mRNAs encoding m2, m3, and m4 subtypes were abundantly expressed in iris, whereas m1 and m5 subtypes were not detected. Selective amplification of the coding regions of m2, m3, and m4 subtypes in iris was carried out using specific...
متن کاملA novel M3 muscarinic acetylcholine receptor is expressed in chick atrium and ventricle.
Prior studies have suggested that heart expresses only the M2 isoform of the muscarinic receptor (Peralta, E.G., Ashkenazi, A., Winslow, J.W., Smith, D.H., Ramachandran, J., and Capon, D.J. (1987) EMBO J. 6, 3923-3929). Tietje and Nathanson (Tietje, K.M., and Nathanson, N. M. (1991) J. Biol. Chem. 266, 17382-17387) have recently demonstrated that the chick heart may be unique since it expresses...
متن کاملAnti-M3 Muscarinic Acetylcholine Receptor Antibodies in Systemic Lupus Erythematosus
Background: Evidences have shown that anti-M3 muscarinic acetylcholine receptor IgG (anti-M3 mAChR IgG) are clinically useful autoantibody that exert a cholinergic pharmacologic effect binding and interacting with M3 mAChR at the level of exocrine gland (salivary and ocular). Aims: The aim of this study was to determine the associations between serum level of anti-M3 mAChR IgG in patients with ...
متن کاملTryptophan-scanning Mutagenesis in the M3 Transmembrane Domain of the Muscle-type Acetylcholine Receptor
Membrane proteins constitute a large fraction of all proteins, yet very little is known about their structure and conformational transitions. A fundamental question that remains obscure is how protein domains that are in direct contact with the membrane lipids move during the conformational change of the membrane protein. Important structural and functional information of several lipid-exposed ...
متن کاملRole of the M3 muscarinic acetylcholine receptor subtype in murine ophthalmic arteries after endothelial removal.
PURPOSE We tested the hypothesis that the M3 muscarinic acetylcholine receptor subtype mediates cholinergic responses in murine ophthalmic arteries after endothelial removal. METHODS Muscarinic receptor gene expression was determined in ophthalmic arteries with intact and with removed endothelium using real-time PCR. To examine the role of the M3 receptor in mediating vascular responses, opht...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m304991200